Et profilbilde

Professor
Cathrine Lillo { "honorific-suffix": "Professor", "fn": "Cathrine Lillo", "tel": "Telephone: +47 51831875", "email": "cathrine.lillo@uis.no" }

Faculty Faculty of Science and Technology
Department Department of Chemistry and Bioscience
Room I8 D-204

 

Research fields

 

Selected publications

Eggen T, Lillo C (2012) The antidiabetic II drug metformin in plants: uptake and translocation to edible parts of cereals, oily seeds, beans, tomato, squash, carrots and potatoes. Journal of Agricultural and Food Chemistry 60, 6929-6935

 

Larbat R, Olsen KM, Slimestad R, Løvdal T, Bénard C, Verheul M, Bourgaud F, Robin C, Lillo C (2012) Influence of repeated short-term nitrogen limitations on leaf phenolics metabolism in tomato. Phytochemistry 77, 119-128

Jonassen EM, Heidari B, Nemie-Feyissa D, Matre P, Lillo C (2011) Protein phosphatase 2A regulatory subunits are starting to reveal their functions in plant metabolism and development. Plant Signaling & Behavior 6, 1216-1218

 

Heidari B, Matre P, Nemie-Feyissa D, Meyer C, Rognli OA, Møller SG, Lillo C (2011) Protein phosphatase 2A B55 and A regulatory subunits interact with nitrate reductase and are essential for nitrate reductase activation. Plant Physiol 156, 144-164

 

Tang W, Yuan M, Wang R, Yang Y, Wang C, Oses-Prieto JA, Kim T-W, Zhou H-W, Deng Z, Gampala SS, Gendron JM, Jonassen EM, Lillo C, DeLong A, Burlingame AL, Sun Y, & Wang Z-Y (2011) PP2A activates brassinosteroid-responsive gene expression and plant growth by dephosphorylating BZR1. Nature Cell Biology 13, 124-131

 

Reumann S, Voitsekhovskaja O, Lillo C (2010) From signal transduction to autophagy of plant cell organelles: lessons from yeast and mammals and plant-specific features. Protoplasma 247, 233-256

 

Løvdal T, Olsen KM, Slimestad R, Verheul M, Lillo C (2010) Synergetic effects of nitrogen depletion, temperature and light on the content of phenolic compounds in leaves of tomato. Phytochemistry 71, 605-613

 

Olsen KM, Heh A, Jugdé H, Slimestad R, Larbat R, Bourgaud F, Lillo C (2010) Identification and characterisation of CYP75A31, a new flavonoid 3'5'-hydroxylase, isolated from Solanum lycopersicum. BMC Plant Biology 10, 21

 

Jolma IW, Laerum OD, Lillo C, Ruoff P (2010) Circadian oscillators in eukaryotes. WIREs System Biology and Medicine. DOI: 10.1002/wsbm.81

 

Jonassen E Müller, Sandsmark B AA, Lillo C (2009) Unique status of NIA2 in nitrate assimilation: NIA2 expression is promoted by HY5/HYH and inhibited by PIF4. Plant Signaling & Behavior 4, 1-3

 

Matre P, Meyer C, Lillo C (2009) Diversity in subcellular targeting of the PP2A B?h subfamily members. Planta 230, 935-945

 

Feyissa DN, Løvdal T, Olsen KM, Slimestad R, Lillo C (2009) The endogenous GL3, but not EGL3 gene is necessary for anthocyanin accumulation as induced by nitrogen depletion in Arabidopsis rosette stage leaves. Planta 230, 747- 754

 

Jonassen EM, Sévin DC, Lillo C (2009) The bZIP transcription factors HY5 and HYH are positive regulators of the main nitrate reductase gene in Arabidopsis leaves, NIA2, but negative regulators of the nitrate uptake gene NRT1.1. J Plant Physiol, 166, 2071-2076

 

Løvdal T, Lillo C (2009) Reference gene selection for quantitative real-time PCR normalization in tomato subjected to nitrogen, cold, and light stress. Analytical biochemistry, 387, 238-242

Olsen KM, Slimestad R, Lea US, Brede C, L¿vdal T, Ruoff P, Verheul M, Lillo C (2009) Temperature and nitrogen effects on regulators and products of the flavonoid pathway: Experimental and kinetic model studies. Plant Cell & Environment 32, 286-299

Lillo C (2008) Signaling cascades integrating light-enhanced nitrate metabolism. Biochem J 415: 11-9

Olsen KM, Lea US, Slimestad R, Verheul M and Lillo C (2008) Differential expression of the four Arabidopsis PAL genes: PAL1 and PAL2 have functional specialization in abiotic environmental triggered flavonoid synthesis. J Plant Physiol 165: 1491-1499

Lillo C, Lea US, Ruoff P (2008) Nutrient depletion as a key factor for manipulating gene expression and product formation in different branches of the flavonoid pathway. Plant Cell and Environment, 31, 587-601

Jonassen EM, Lea US, Lillo C (2008) HY5 and HYH are positive regulators of nitrate reductase in seedlings and rosette stage leaves. Planta 227: 559-564

Lea US, Slimestad R, Smedvig P, Lillo C (2007) Nitrogen deficiency enhances expression of specific MYB and bHLH transcription factors and accumulation of end products in the flavonoid pathway. Planta 225(5) 1245-1253

Lea US, Leydecker M-T, Quilleré I, Meyer C, Lillo C (2006) Posttranslational regulation of nitrate reductase strongly affects the levels of free amino acids and nitrat, whereas transcriptional regulation has only minor influence. Plant Physiol 140: 1085-1094

Provan F, Haavik J, Lillo C (2006) The regulatory phosphorylated serine in full-length nitrate redutase is necessary for optimal binding to a 14-3-3 protein. Plant Science, 170: 394-398

Lillo C,  Jørgensen KB (2005) Summary from: Workshop on growing plants for increased nutritional value. Reports from the University of Stavanger Nr.1 Workshop report

Meyer C, Lea US, Provan F, Kaiser WM, Lillo C (2005) Is nitrate reductase a major player in the plant NO game? Photosynthesis Research 83: 181-189

Lillo C, Meyer C, Lea U, Provan F, Oltedal S, (2004) Mechanism and importance of post-translational regulation of nitrate reductase, J Exp Bot 55: 1-8

Christensen MK, Falkeid G, Loros JJ, Dunlap JC, Lillo C, Ruoff P (2004) A nitrate induced frq-less oscillator in Neurospora crassa. J Biol Rhythms 19(4): 280-286

Lea US, ten Hoopen F, Provan F, Kaiser WM, Meyer C, Lillo C (2004) Mutation of the regulatroy phosphorylation site of tobacco nitrate reductase results in high nitrite excretion and NO emission from leaf and root tissue. Planta 219: 59-65

Lillo C (2004) Light regulation of nitrate uptake, assimilation and metabolism. In :Plant Ecophysiology, Vol 3 Nitrogen Acquisition and Assimilation in Higher Plants (eds. Amancio S, and Stulen I) Chapter 6, Kluwer Academic Publisher, Dordrecht, 2004, pp149- 184

Lillo C, Lea US, Leydecker M-T, Meyer C (2003) Mutation of the regulatory phosphorylation site of tobacco nitrate reductase results in constitutive activation of the enzyme in vivo and nitrite accumulation. Plant J 35(5): 566-573.

Lillo C and Meyer C (2001) Biological Clocks and the Nitrate Reductase Oscillating System. Journal of Biological Rhythm Research. Biol Rhythm Res 32: 489-500

Lillo C, Meyer C, Ruoff P (2001) Multiple Oscillatory Feedback Loops. The Central Clock Dogma Contra Multiple Oscillatory Feedback Loops. Plant Physiol 125: 1554-1557

Appenroth K-L, Rezarta M, Jourdan V, Lillo C (2000) Phytochrome and post-translational regulation of nitrate reductase. Plant Science 159: 51-56.

Provan F, Aksland L-M, Meyer C, Lillo C (2000) Deletion of the nitrate reductase N-terminal domain still allows binding of 14-3-3 proteins but affects their inhibitoryproperties. Plant Physiol. 123: 757-764.

Lillo C, Kazaic S, Ruoff P, and Meyer C (1997) Effects of 14-3-3 proteins on NR in light and darkness. Plant Physiol. 114: 1377-1383

Lillo C, Smith LH, Nimmo HG and Wilkins MB (1996) Light/dark regulation of nitrate reductase and phosphoenol carboxylase in barley protoplasts. Planta 200: 180-185

MacKintosh C, Douglas P and Lillo C(1995) Identification of a protein that inhibits the phosphorylated form of nitrate reductase from spinach leaves. Plant Physiology 107: 451-457.

Lillo C (1994) Light regulation of nitrate reductase in green leaves of higher plants. Minireview. Phys. Plant. 90: 616-620

Lillo C and Olsen JE (1989) Growth and shoot formation in protoplast-derived calli of Brassica oleracea ssp. acephala and ssp.capitata. Plant Cell, Tissue and Organ Culture 17:91-100.

Lillo C and Shahin AS 1986 Rapid regeneration of plants from hypocotyl protoplasts and root segments of cabbage. HortScience 21(2):315-317

Research in progress

Research

The main research interest of the group headed by Prof Lillo is signalling to nitrogen and flavonoid metabolism. We aim at understanding how environmental factors (light and temperature) lead to changes in gene expression and post-translational regulation of enzymes in nitrogen and flavonoid metabolism. An important aspect is how signals between different organelles and compartments of the cell integrate to control these metabolic pathways.

Regulation of nitrogen assimilation and involvement of protein phosphatases

Nitrogen is a key input factor in agriculture, often the limiting component for growth, and therefore of great economic interest. To be able to improve the efficiency of nitrogen uptake and assimilation, a solid knowledge of the cellular processes involved are necessary. To extend the current knowledge we work on regulation mechanisms on the transcriptional as well as post-transcriptional level.

Previous research results, and our current research, are in favour of protein phoshatases being important in activation of a key enzyme in nitrate assimilation, nitrate reductase, at the transcriptional as well as post-translational level (Lillo 2008). Canonical PP2A (Protein phosphatase 2A) enzymes are heterotrimeric proteins consisting of a catalytic subunit (C), a regulatory subunit (B), and a scaffolding subunit (A). In Arabidopsis thaliana there are at least 20 scaffolding/regulatory subunits, and their specific function in metabolism and physiology is hardly known. We are working on identifying the PP2A subunits interacting with nitrate reductase. When these subunits are identified we can proceed into more details concerning upstream components, and we hope to be able to understand how signals are transmitted form the chloroplasts to activate nitrate reductase in the cytosol.

Nitrate reductase can serve as a model system when investigating protein phosphatases since an easy assay is available for testing phosphorylation and dephosphorylation of this enzyme. Because basic knowledge is generally lacking concerning the physiological functions of different PP2As, the project will no doubt also lead to many interesting spin-off discoveries on the function of protein phosphatases. For reviews see Lillo et al. 2004, and Lillo 2008.

Nitrogen and temperature as key factors for regulation and product formation in the flavonoid pathway

Flavonoids are considered to important for human health as well as for the resistance of plants towards pathogens and abiotic stress. The overall long-term aim of this project is to increase the quality of fruit and vegetables by manipulating growth conditions. The project is in collaboration with Bioforsk at Særheim research station.

Flavonoids are formed in leaves in response to UV-light, nutrient depletion, and low temperature (Olsen et al. 2009). The physiological function of flavonoids in abiotic stress responses is not well understood, but flavonoids appear to be important for better tolerance of limiting nitrogen conditions. Synthesis of flavonoids are regulated by at least four classes of transcription factors in higher plants MYB, bHLH, WD40-repeat, and bZIP. To understand the influence of environmental factors at a molecular level we are testing expression profiles of regulators as well as genes encoding enzymes of the pathway.

We have been using Arabidopsis as a model plant, but are currently extending our research to tomato. For a review see Lillo et al. 2008.

Please go to this site for further information.

 

References

Olsen KM, Slimestad R, Lea US, Brede C, Løvdal T, Ruoff P, Verheul M, Lillo C (2009) Temperature and nitrogen effects on regulators and products of the flavonoid pathway: Experimental and kinetic model studies. Plant Cell & Environment 32, 286-299

Lillo C (2008) Signalling cascades integrating light-enhanced nitrate metabolism. Biochem J, 415: 11-9

Lillo C, Meyer C, Lea U, Provan F, Oltedal S (2004) Mechanism and importance of post- translational regulation of nitrate reductase. J Exp Bot: 55: 1275-1282

 

Norwegian cooperations

Dr. Michel Verheul (Bioforsk, Særheim)

Dr. Rune Slimestad (PlantChem, Særheim)

and CORE professors at UiS

 

International cooperations

Dr. Christian Meyer at INRA, Versailles, France

Prof. Fréderic Bourgaud and collaborators at the University of Nancy/INRA, France

Work experience

 

Scientific publications (from Cristin)

  • Eggen, Trine; Lillo, Cathrine (2016). Role of transporters for organic cations in plants for environmental cycling of pharmaceutical residues. Springer International Publishing. ISBN 978-3-319-23792-3. 13 p.
  • Jørgensen, Kåre B.; Lillo, Cathrine (2005). First International Workshop on Growing Plants for Increased Nutritional Value. Universitetet i Stavanger. ISBN 8276442692. 38 p.
  • Averkina, Irina Orestovna; Creighton, Maria Terese; Nemie-Feyissa, Dugassa; Kataya, Amr Ramzy Abass; Lillo, Cathrine (2017). Protein phosphatase 2A activity in various Arabidopsis mutants. BioCat; 2017-06-12 - 2017-06-14.
  • ElShobaky, Ahmed; Lillo, Cathrine; Kataya, Amr Ramzy Abass (2017). Investigating the role of PP2A-B’ζ in regulating energy metabolism in mitocondria..
  • Kataya, Amr Ramzy Abass; ElShobaky, Ahmed; Lillo, Cathrine (2017). Trehalose-6-phosphate phosphatase I harbors a novel peroxisomal signal and is implicated in abiotic stress and development. 2017-06-19 - 2017-06-23.
  • Kolton, Anna; Creighton, Maria Terese; Wisniexska, Alina; Lillo, Cathrine; Czernicka, Malgorzata (2017). PP2A-C subunits and other parameters under hypoxia stress in tomato leaves and roots. Polish Society of Experimental Plant Biology; 2017-09-12 - 2017-09-15.
  • Yuan, Guoliang; Heidari, Behzad; de Storme, Nico; Scnitter, Arp; Lillo, Cathrine; Geelen, Danny (2017). Protein phosphatse 2A protects centromeric sister chromatid cohesion in Arabidopsis meiosis I by maintaining REC8 at the chromocenters. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforsc; 2017-03-30 - 2017-03-31.
  • Kataya, Amr Ramzy Abass; Heidari Ahootapeh, Behzad; Lillo, Cathrine (2015). Protein phosphatase 2A regulatory subunits affecting plant innate immunity, energy metabolism, and flowering time – joint functions among B'η subfamily members.
  • Kataya, Amr Ramzy Abass; Lillo, Cathrine (2015). Investigating Protein Phosphatase 2A Regulatory Subunits: From Subcellular Localization to Abiotic Stress Implications.. 2015-06-29 - 2015-07-01.
  • Kataya, Amr Ramzy Abass; Schei, Edit; Lillo, Cathrine (2015). Peroxisomal Protein Phosphatases: Identifications and Biotic Stress Implications. 2015-07-02 - 2015-07-05.
  • Kataya, Amr Ramzy Abass; Heidari Ahootapeh, Behzad; Lillo, Cathrine (2013). B’θ regulatory subunit of protein phosphatase 2a is involved in peroxisomal fatty acid β-oxidation, flowering, and plant innate immunity.. 2013-07-04 - 2013-07-07.
  • Heidari, Behzad; Jonassen, Else Muller; Nemie-Feyissa, Dugassa; Lillo, Cathrine (2011). Role of Protein phosphatase 2A in adaptation mechanisms of higher plants. CNRS - Conférences Jacques Monod; 2011-04-30 - 2011-05-04.
  • Heidari, Behzad; Matre, Polina Rafaelevna; Jonassen, Else Muller; Nemie-Feyissa, Dugassa; Meyer, C; Rognli, OA; Møller, Simon Geir; Lillo, Cathrine (2011). Protein phosphatase 2A regulatory subunits are essential for metabolism and plant development. 2011-08-22 - 2011-08-25.
  • Jolma, Ingunn Westvik; Lillo, Cathrine; Ruoff, Peter (2011). Temperature dependence of rutin-Fe(II) binding constants determined by isothermal titration calorimetry. SPPS; 2011-08-20 - 2011-08-25.
  • Lillo, Cathrine (2011). Anthocyanins. Måltidets hus;
  • Lillo, Cathrine (2011). Biotechnology research at CORE.
  • Lillo, Cathrine (2011). Molecular farming. Måltidets hus;
  • Lillo, Cathrine (2011). Small RNAs and control of gene expression. Måltidets hus;
  • Jonassen, Else Müller; Matre, Polina; Hidary, Behzad; Feyissa, Dugassa N.; Rognli, Odd Arne; Lillo, Cathrine; Lillo, Cathrine (2010). PROTEIN PHOSPHATASE 2A REGULATORY B? SUBUNITS ESSENTIAL FOR SEED SET AND GROWTH. 2010-11-11.
  • Jonassen, Else Müller; Matre, Polina; Hidary, Behzad; Feyissa, Dugassa N.; Rognli, Odd Arne; Lillo, Cathrine; Lillo, Cathrine (2010). PROTEIN PHOSPHATASE 2A SUBUNITS IMPORTANT FOR ACTIVATING KEY ENZYMES OF PRIMARY METABOLISM, AND FOR STIMULATING GROWTH THROUGH THE BRASSINOSTEROID SIGNALLING CASCADE. 2010-10-06 - 2010-10-09.
  • Lillo, Cathrine (2009). Nitrogen depletion as a key factor for gene expression and product formation in different branches of the flavonoid pathway. 2007-07-27 - 2009-07-31.
  • Jonassen, Else Müller; Lillo, Cathrine (2008). Light-enhanced nitrate assimilation involves phytochrome, HY5/HYH and signalling from chloroplasts. 2008-06-04 - 2008-06-08.
  • Lillo, Cathrine (2007). Influence of Nitrogen Depletion and Temperature on Flavonoid Metabolism in Arabidopis thaliana. 2007-09-12 - 2007-09-14.
  • Olsen, Kristine Marie; Lea, Unni Synnøve; Slimestad, Rune; Verheul, Michel; Lillo, Cathrine (2007). Differential expression of the four Arabidopsis PAL genes. 2007-09-12 - 2007-09-14.
  • Lea, Unni Synnøve; Slimestad, Rune; Lillo, Cathrine (2006). Nitrogen deficiency enhances expression of specific MYB and bHLH transcription factors and accumulation of end products in the flavonoid pathway. 2006-07-17 - 2006-07-21.
  • Lillo, Cathrine (2006). Regulation of nitrate reductase to optimize nitrogen assimilation and avoid toxic products like nitrate and nitric oxide. 2006-02-01.
  • Lillo, Cathrine; Meyer, Christian; Lea, Unni Synnøve (2005). NItrate reductase and biological clocks. 2005-05-12 - 2005-05-14.
  • Lillo, Cathrine (2004). Mechanisms and importance of post-translational regulation of nitrate reductase. 2004-06-23 - 2004-06-27.
  • Lillo, Cathrine; Meyer, Christian; Lea, Unni Synnøve (2004). Regulation of nitrate reductase activity. 2004-08-23 - 2004-08-27.
  • Appenroth, K.-J.; Lillo, Cathrine (2003). Nitrat reduktase - Shclüsselenzym der Nitratassimilation Nitrate reducase - Key enzyme of nitrogen metabolism.
  • Lea, Unni Synnøve; Meyer, Christian; Lillo, Cathrine (2003). Nitrate reductase and biological clocks. 2003-05-11 - 2003-05-16.
  • Lillo, Cathrine; Meyer, Christian; Lea, Unni Synnøve (2003). Mutation of the regulatory phosphorylation site of tobacco nitrate reductase results in constitutive activation of the enzyme in vivo and nitrite accumulation. 2003-06-23 - 2003-06-28.
  • Lillo, Cathrine; Kazazic, Sabina; Ruoff, Peter; Meyer, C. (1999). Regulation of nitrate reductase in light and darkness, effects of a signalling protein.
  • Lillo, Cathrine; Provan, Fiona (1999). Deletion of an acidic stretch in the amino terminal domain of Nicotiana nitrate reductase does not prevent binding of Nicotiana 14-3-3 protein but influence on some characteristics of the NR/14-3-3 complex.
  • Provan, Fiona; Lillo, Cathrine; Meek, Sarah; MacKintosh, Carol (1999). Nutrient and hormonal regulation of interactions between 14-3-3 proteins and binding targets in arabidopsis cell culture.
  • Savino, Gil; Pigaglio, Emmanuelle; Crete, Patrice; Lillo, Cathrine; Meyer, Christian (1999). Regulation of nitrate assimilation.
  • Lillo, Cathrine; Smith, L.; Nimmo, H.G.; Wilkins, M.B. (1998). Light/dark regulation in leaves and protoplasts of higher plants.
  • Pigaglio, E.; Lillo, Cathrine; Meyer, C. (1998). Post-translational regulation of nitrate reductase in Nicotiana.
  • Pigaglio, E.; Lillo, Cathrine; Meyer, C. (1998). Post-translational regulation of nitrate reductase in Nicotiana.
  • Smith, L.; Lillo, Cathrine; Nimmo, H.G.; Wilkins, M.B. (1998). Coordination of carbon and nitrogen metabolism and the regulation of phosphoenol pyruvate in C3 plants.