Et profilbilde

Førsteamanuensis
Clemens Furnes { "honorific-suffix": "Førsteamanuensis", "fn": "Clemens Furnes", "tel": "Telefon: 51831867", "email": "clemens.furnes@uis.no" }

Avdeling/enhet Det teknisk- naturvitenskapelige fakultet
Institutt/senter Institutt for matematikk og naturvitenskap
Rom I8 D-212

Undervisning

Forskningsområder

ArcticGlue

STAMP

PITS

Bioeconomy

Utvalgte publikasjoner

Mork-Jansson AE, Gargano D, Kmiec K, Furnes C, Shevela D, Eichacker LA. Lil3 dimerization and chlorophyll binding in Arabidopsis thaliana. FEBS Lett. 2015 Oct 7;589(20 Pt B):3064-70.

Mork-Jansson, E.A., Bue, K.A., Gargano, D., Furnes, C., Reisninger, V., Arnold, J., Kmiec, K., Eichacker, A.l. Lil3 assembles with proteins regulating chlorophyll biosynthesis in barley. PLOS One. 2015 Jul 14;10 (7).

Aksnes, H., Damme, V.P., Starheim, k., Marie, M., Hoel, C., Kalvik, V.t., Hole, K., Glomnes, N., Ljostveit, S., Furnes, C., Ziegler, M., Niere, M., Gevaert, K., Arnesen, T. An organellar N?-acyltransferase Naa60 acetylates cytosolic N-termini of transmembrane proteins and maintains Golgi integrity. 2015 Mar 3;10(8):1362-74.

Furnes C, Kileng Ø, Jensen I, Robertsen B. Isolation and characterization of two cDNAs encoding transglutaminase from Atlantic cod (Gadus morhua). 2014 Jan; 36(1): 276-283.

Hynes N, Furnes C, Winther T, Larsen A, Fredriksen B, Bogwald J. and Dalmo R. Immune response of Atlantic salmon to recombinant flagellin. 2011 Oct; 29(44): 7678-87.

Furnes C and Robertsen B. Molecular cloning and characterization of bloodthirsty from Atlantic cod (Gadus morhua). Fish Shellfish Immunol. 2010 Dec; 29(6):903-9.

Furnes C, Kileng Ø, Rinaldo CH, Seppola M, Jensen I, Robertsen B. Atlantic cod (Gadus morhua L.) possesses three homologues of ISG15 with different expression kinetics and conjugation properties. Dev Comp Immunol. 2009 Dec; 33(12):1239-46.

Furnes C, Seppola M, Robertsen B. Molecular characterisation and expression analysis of interferon gamma in Atlantic cod (Gadus morhua). Fish Shellfish Immunol. 2009 Feb;26 (2):285-92.

Furnes C, Andresen V, Szilvay AM. Functional rescue of an oligomerization-defective HIV-1 Rev mutant by fusion with an oligomeric tag. Arch Virol. 2008;153(2):357-62.

Furnes C, Arnesen T, Askjaer P, Kjems J, Szilvay AM. HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain. Retrovirology. 2005 Jun 10;2:39.






Pågående forskning


ArcticGlue


Transglutaminases (TGs) are a family of enzymes that catalyzes cross-linking of proteins by forming covalent bonds between lysine residues and glutamine residues in different polypeptides. TGs have great potential for the food industry because the isopeptide bonds created by TGs exhibit high resistance to proteolytic degradation (proteolysis) and bind a range of proteins that are not normally linked. Once the binding has occurred, food products can therefore withstand further processing such as cooking, smoking, freezing or chilling. We have sequenced and cloned a transglutaminase which is cold-adapted, that is it has its activity optimum at a low temperature of. This enzyme is particular useful in food industry, because processing at this temperature does not diminish the quality of the product. A large volume of bi-products from the Norwegian fish industry may in this manner be restructured to new and attractive products with a higher value for the producer, and it will the consumption of fish products in Norway. The focus of this project will be to develop a commercial product that can be used as a biological adhesive with unlimited potential for use in the food industry and cooking. This will be achieved by large-scale production and purification of this recombinant enzyme.


 


How do proteins move and interact around the cell: ordered or random?


Localization of cellular proteins can be studied by immunocytochemistry in fixed cells. A static fluorescence microscopy image usually reflects the steady state localization of cellular proteins but is not suitable to reveal sites where proteins localize transiently or the order of transit through the different cellular compartments. Studies of trafficking of proteins inside the cells have been improved by the use of proteins fused to green fluorescent protein (GFP), which permits direct observation of GFP fusions proteins in living cells. However, the GFP-technology has the disadvantage that the method only allows monitoring of GFP-fusion protein movements in the initial phase following initiation of expression/visualization. After a while it will not be possible to determine the origin of the protein that produces the fluorescent signal. In order to overcome the limitations posed above a new approach called STAMP (Subcellular Trafficking Assayed by Means of Proteolytic cleavage) is being developed to monitor if a protein has ?visited? a specific cellular compartment. The availability of a method for detecting transient protein interaction is also lacking and an method is being developed in our lab named PITS (Protein interaction Trapping system) to overcome this limitation. The availability of a generalized method for monitoring ?visits? to different subcellular compartments and detecting transient protein interactions would serve useful additions to the tool chest for both cell and cancer research.


Arbeidserfaring

2014?d.d. Universitetet i Stavanger, Senter for organelleforskning, forsker/prosjektleder

2010?2014 Universitetet i Stavanger, Senter for organelleforskning, post doc.

2005?2010 Universitetet i Tromsø, Fakultet for biovitenskap, fiskeri og økonomi, post doc. / forsker

1998?1999 Universitetet i Tromsø, institutt for medisinsk biologi, vitenskapelig assistent ved avdeling for bioteknologi for Biotec ASA

Vitenskapelige publikasjoner (fra CRISTin)

  • Furnes, Clemens; Szilvay, Anne Marie; Aasland, Rein (2005). Subcellular localization, oligomerization and trafficking of HIV-1 Rev. Universitetet i Bergen. ISBN 82-308-0020-0. 85 s.
  • Eichacker, Lutz Andreas; Mork-Jansson, Astrid Elisabeth; Bue, Ann Kristin; Gargano, Daniela; Arnold, Janine; Reisinger, Veronika; Kmiec, Karol; Furnes, Clemens; Shevela, Dmitriy (2016). Chlorophyll binding to light-harvesting-like protein 3. Thorsten Hamann; 2016-06-15 - 2016-06-17.
  • Eichacker, Lutz Andreas; Mork-Jansson, Astrid Elisabeth; Gargano, Daniela; Bue, Ann Kristin; Arnold, Janine; Reisinger, Veronika; Furnes, Clemens (2016). Lil3 and the protein’s function in chlorophyll synthesis.. Poul Erik Jensen; 2016-10-25 - 2016-10-28.
  • Furnes, Clemens; Mannelqvist, Monica; Sarria, Shirley Vanessa; Aasland, Rein; Szilvay, Anne Marie (2016). In vivo proteolytic activity assayed by subcellular localization switching. Norsk Biokjemisk selskap; 2016-01-21 - 2016-01-24.
  • Furnes, Clemens (2014). Arctic Glue. Renaissance Tuscany II Ciocco Resort; 2014-06-29 - 2014-07-04.
  • Furnes, Clemens (2014). Arctic Glue. Renaissance Tuscany II Ciocco Resort; 2014-06-29 - 2014-07-04.
  • Furnes, Clemens (2014). Fra lab-benk til produkt. 2014-12-10.
  • Furnes, Clemens (2014). Fra olje til bioøkonomi: en ny, smart og grønn teknologi. 2014-11-07.
  • Furnes, Clemens (2014). iFood. 2014-10-30.
  • Furnes, Clemens (2013). ArcticGlue. 2013-12-16.
  • Furnes, Clemens (2013). Haikerens guide til biokjemi. 2013-03-12.
  • Furnes, Clemens (2012). Innovative enzyme technology: development of a new glue enzyme for use in food industry. 2012-04-13.
  • Furnes, Clemens (2012). Limeenzym fra fisk. 2012-09-26.
  • Furnes, Clemens (2012). Recombinant expression and interaction partner study of Lil3, a light harvesting-like protein. Cold Spring Harbor; 2012-05-30 - 2012-06-04.
  • Furnes, Clemens (2011). Immune response of Atlantic salmon to recombinant flagellin. 2011-06-24.
  • Furnes, Clemens; Arnesen, Thomas; Askjaer, Peter; Kjems, Jørgen; Szilvay, Anne Marie (2004). Activity restoration of the HIV-1 Rev multimerization mutant M4 by addition of an extra basic domain. Cold Spring Harbor Laboratory; 2004-05-25 - 2004-05-30.
  • Arnesen, Thomas; Furnes, Clemens; Askjaer, Peter; Kjems, Jørgen; Szilvay, Anne Marie (2001). Six Rev monomers binding to the HIV-1 mRNA is not sufficient for complete Rev activity. 2001-10-26 - 2001-10-28.
  • Furnes, Clemens; Askjaer, Peter; Kjems, Jørgen; Szilvay, Anne Marie (2001). The role of oligomerization in nuclear import and function of HIV-1 Rev. 2001-05-22 - 2001-05-27.
  • Furnes, Clemens; Szilvay, Anne Marie (2000). THE FUNCTIONAL ROLE OF HIV-1 REV OLIGOMERIZATION. 2000-09-13 - 2000-09-17.
  • Furnes, Clemens; Aasland, Rein; Szilvay, Anne Marie (2000). Subnuclear localization assessed by protein cleavage. 2000-08-23 - 2000-08-24.