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This is the study programme for 2019/2020. It is subject to change.


Basic kinetics and enzyme kinetics, elementary reactions, diffusion-controlled reactions, UV-visible and fluorescence spectroscopy, fluorescence microscopy. Environmental parameters and their influence on chemical/enzymatic reactions. Single and multi-substrate enzyme kinetics, allosteric proteins, regulation of biological activity, biological rhythms. Use of spreadsheet and programming in physical and biophysical chemistry.

Learning outcome

After having completed the course the student should be able to:
  • apply the principles in kinetics/enzyme kinetics
  • calculate life time of unstable compounds on basis of first-order kinetic half-life
  • experimentally determine KM and Vmax values for enzyme catalyzed reactions
  • understand the basics of allosteric regulation of biochemical and metabolic pathways
  • estimate diffusion times
  • know the principles of an ultracentrifuge
  • determine binding constants
  • understand the principles of reversible inhibition
  • show how pH influences enzyme-catalyzed reactions
  • apply the principles of UV-VIS absorption and fluorescence spectroscopy in determining the amount of substances
  • understand the principles and applications of FRET
  • determine binding constants from isothermal calorimeter experiments
  • estimate entropy and enthalpy contributions to binding constants
  • use open access programs, such as Berkeley Madonna, for mathematical modelling of biochemical reactions and processes.

After having completed the course successfully, the student has a good competence/basis to work with a Master thesis or doing chemical, biochemical or molecular biology related research.

Contents

Basic kinetics and enzyme kinetics, elementary reactions, diffusion-controlled reactions, UV-visible and fluorescence spectroscopy, fluorescence microscopy. Environmental parameters and their influence on chemical/enzymatic reactions. Single and multi-substrate enzyme kinetics, allosteric proteins, regulation of biological activity. Use of spreadsheet and programming in physical and biophysical chemistry.

Required prerequisite knowledge

None.

Recommended previous knowledge

General chemistry at university level. Knowledge within biochemistry is an advantage.

Exam

Weight Duration Marks Aid
Written exam1/14 hoursA - F1)
Compilation of mathematical formulae (Rottmann).
1) Approved, basic calculator

Coursework requirements

Laboratory teaching, PC-exercises.
Compulsory course attendance that must be completed and approved before access to the laboratory: Electronic Course in Health, Safety and Environment
Mandatory lab assignments are to be completed at the times and in the groups that are assigned and published. Absence due to illness or other reasons must be communicated to the laboratory personnel as soon as possible. One cannot expect that provisions for completion of the lab assignments at other times can be made, unless prior arrangements with the laboratory personnel have been agreed.
Failure to complete the assigned labs on time or not having them approved will result in barring from taking the course exam.

Course teacher(s)

Coordinator laboratory exercises
Xiang Ming Xu
Course teacher
Heinz Peter Ruoff
Head of Department
Gro Johnsen

Method of work

Four hours lectures and six hours practical lab work each week.

Overlapping courses

Course Reduction (SP)
Physical Chemistry (MOT450_1) 10
Biophysical Chemistry (MBI110_1) 10

Open to

Master level studies at the Faculty of Science and Technology.

Course assessment

Forms and/or discussion

Literature

The detailed curriculum will be given during the first lecture, and will be available on the learning platform Canvas. The curriculum is based on various sources (books, lecture notes), of which some are posted on Canvas.
Chang, Raymond; "Physical chemistry for the biosciences", 2005.
University science books, Sausalito, California, USA. ISBN 1-891389-33-5
Castellan can be used as a supportive text.
A comprehensive list of planned lectures and additional supporting
literature/handouts on Canvas: file: "Lectures overview"


This is the study programme for 2019/2020. It is subject to change.

Sist oppdatert: 15.12.2019