- apply the principles in kinetics/enzyme kinetics
- calculate life time of unstable compounds on basis of first-order kinetic half-life
- experimentally determine KM and Vmax values for enzyme catalyzed reactions
- understand the basics of allosteric regulation of biochemical and metabolic pathways
- estimate diffusion times
- know the principles of an ultracentrifuge
- determine binding constants
- understand the principles of reversible inhibition
- show how pH influences enzyme-catalyzed reactions
- apply the principles of UV-VIS absorption and fluorescence spectroscopy in determining the amount of substances
- understand the principles and applications of FRET
- determine binding constants from isothermal calorimeter experiments
- estimate entropy and enthalpy contributions to binding constants
- use open access programs, such as Berkeley Madonna, for mathematical modelling of biochemical reactions and processes.
After having completed the course successfully, the student has a good competence/basis to work with a Master thesis or doing chemical, biochemical or molecular biology related research.
Required prerequisite knowledge
Recommended previous knowledge
|Written exam||1/1||4 hours||A - F||1)|
Compilation of mathematical formulae (Rottmann).
Mandatory lab assignments are to be completed at the times and in the groups that are assigned and published. Absence due to illness or other reasons must be communicated to the laboratory personnel as soon as possible. One cannot expect that provisions for completion of the lab assignments at other times can be made, unless prior arrangements with the laboratory personnel have been agreed.
Failure to complete the assigned labs on time or not having them approved will result in barring from taking the course exam.
- Coordinator laboratory exercises
- Xiang Ming Xu
- Course teacher
- Heinz Peter Ruoff
- Head of Department
- Gro Johnsen
Method of work
|Physical Chemistry (MOT450_1)||10|
|Biophysical Chemistry (MBI110_1)||10|
Chang, Raymond; "Physical chemistry for the biosciences", 2005.
University science books, Sausalito, California, USA. ISBN 1-891389-33-5
Castellan can be used as a supportive text.
A comprehensive list of planned lectures and additional supporting
literature/handouts on Canvas: file: "Lectures overview"
Sist oppdatert: 27.01.2020