Basic kinetics and enzyme kinetics, elementary reactions, diffusion-controlled reactions, UV-visible and fluorescence spectroscopy, fluorescence microscopy. Environmental parameters and their influence on chemical/enzymatic reactions. Introduction to reaction-kinetic modelling. Single and multi-substrate enzyme kinetics, allosteric proteins, regulation of biological activity, biological rhythms. Use of spreadsheet and programming in physical and biophysical chemistry.
Course description for study year 2022-2023. Please note that changes may occur.
After having completed the course the student should be able to:
apply the principles in kinetics/enzyme kinetics
calculate life time of unstable compounds on basis of first-order kinetic half-life
experimentally determine KM and Vmax values for enzyme catalyzed reactions
understand the basics of allosteric regulation of biochemical and metabolic pathways
estimate diffusion times
can do basic reaction-kinetic modelling with Matlab
determine binding constants
understand the principles of reversible inhibition
show how pH influences enzyme-catalyzed reactions
apply the principles of UV-VIS absorption and fluorescence spectroscopy in determining the amount of substances
understand the principles and applications of FRET
After having completed the course successfully, the student has a good competence/basis to work with a Master thesis or doing chemical, biochemical or molecular biology related research.
Basic kinetics and enzyme kinetics, elementary reactions, diffusion-controlled reactions, UV-visible and fluorescence spectroscopy, fluorescence microscopy. Environmental parameters and their influence on chemical/enzymatic reactions. Single and multi-substrate enzyme kinetics, allosteric proteins, regulation of biological activity. Use of spreadsheet and programming in physical and biophysical chemistry.
Required prerequisite knowledge
General chemistry at university level. Knowledge within biochemistry is an advantage.
Form of assessment
- 1), Compilation of mathematical formulae (Rottmann)
1) Approved, basic calculator
Laboratory teaching, PC-exercises.
Compulsory course attendance that must be completed and approved before access to the laboratory: Electronic Course in Health, Safety and Environment
Mandatory lab assignments are to be completed at the times and in the groups that are assigned and published. Absence due to illness or other reasons must be communicated to the laboratory personnel as soon as possible. One cannot expect that provisions for completion of the lab assignments at other times can be made, unless prior arrangements with the laboratory personnel have been agreed.
Failure to complete the assigned labs on time or not having them approved will result in barring from taking the course exam.