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Biophysical Chemistry BIO500

Basic kinetics and enzyme kinetics, elementary reactions, diffusion-controlled reactions, UV-visible and fluorescence spectroscopy, fluorescence microscopy. Environmental parameters and their influence on chemical/enzymatic reactions. Introduction to reaction-kinetic modelling. Single and multi-substrate enzyme kinetics, allosteric proteins, regulation of biological activity, biological rhythms. Use of spreadsheet and programming in physical and biophysical chemistry.


Course description for study year 2021-2022

Facts
Course code

BIO500

Version

1

Credits (ECTS)

10

Semester tution start

Autumn

Number of semesters

1

Exam semester

Autumn

Language of instruction

English

Offered by

Faculty of Science and Technology, Department of Chemistry and Bioscience

Learning outcome

After having completed the course the student should be able to:

  • apply the principles in kinetics/enzyme kinetics
  • calculate life time of unstable compounds on basis of first-order kinetic half-life
  • experimentally determine KM and Vmax values for enzyme catalyzed reactions
  • understand the basics of allosteric regulation of biochemical and metabolic pathways
  • estimate diffusion times
  • can do basic reaction-kinetic modelling with Matlab
  • determine binding constants
  • understand the principles of reversible inhibition
  • show how pH influences enzyme-catalyzed reactions
  • apply the principles of UV-VIS absorption and fluorescence spectroscopy in determining the amount of substances
  • understand the principles and applications of FRET

After having completed the course successfully, the student has a good competence/basis to work with a Master thesis or doing chemical, biochemical or molecular biology related research.

Content
Basic kinetics and enzyme kinetics, elementary reactions, diffusion-controlled reactions, UV-visible and fluorescence spectroscopy, fluorescence microscopy. Environmental parameters and their influence on chemical/enzymatic reactions. Single and multi-substrate enzyme kinetics, allosteric proteins, regulation of biological activity. Use of spreadsheet and programming in physical and biophysical chemistry.
Required prerequisite knowledge
None
Recommended prerequisites
General chemistry at university level. Knowledge within biochemistry is an advantage.
Exam
Form of assessment Weight Duration Marks Aid
Written exam 1/1 4 Hours A - F 1), Compilation of mathematical formulae (Rottmann) 1)

1) Approved, basic calculator

Coursework requirements
Laboratory teaching, PC-exercises.

Compulsory course attendance that must be completed and approved before access to the laboratory: Electronic Course in Health, Safety and Environment

Mandatory lab assignments are to be completed at the times and in the groups that are assigned and published. Absence due to illness or other reasons must be communicated to the laboratory personnel as soon as possible. One cannot expect that provisions for completion of the lab assignments at other times can be made, unless prior arrangements with the laboratory personnel have been agreed.

Failure to complete the assigned labs on time or not having them approved will result in barring from taking the course exam.

Course teacher(s)
Course coordinator: Heinz Peter Ruoff
Course teacher: Sittana Babiker Hassan Matar
Head of Department: Ingunn Westvik Jolma
Course teacher: Heinz Peter Ruoff
Method of work
Four hours lectures and six hours practical lab work each week.
Open for
Biological Chemistry - Master of Science Degree Programme Exchange programme at Faculty of Science and Technology
Course assessment
Forms and/or discussion
Overlapping courses
Course Reduction (SP)
Physical Chemistry (MOT450) 10
Biophysical Chemistry (MBI110) 10
Literature
The syllabus can be found in Leganto