In a new research paper published in the journal PlosOne, professor Lutz Eichacker and postdoc Astrid Mork-Jansson at CORE provide the proof that a protein called Lil3 which has been found earlier to be key to the process is binding chlorophyll.
In barley plants germinating in the darkness of soil, the Lil3 protein accumulates when the leaf perceives the first light and the first chlorophyll molecules are made.
Crucial to photosynthesis
In contrast, plants were struggling to survive, and accumulation of chlorophyll and of the photosynthetic machinery failed, when the Lil3 gene was removed from the genome.
It appeared that binding of chlorophyll by LiL3 could be a crucial step in the development of photosynthesis.
Binds chlorophyll 1:1
In the work now published, the researchers cloned the Lil3 gene and synthesized the Lil3 protein in the bacterium Escherichia coli. When they isolated the Lil3 protein and incubated it in the test tube with chlorophyll, they found that Lil3 binds one molecule of chlorophyll per protein.
Follow-up research will investigate further how LIL3 uses the bound chlorophyll to promote building the photosynthetic machinery.
Text and photo: Leiv Gunnar Lie
Paper: Characterization of chlorophyll binding to LIL3. Mork-Jansson AE, Eichacker LA. PLoS One. 2018 Feb 1;13(2):e0192228. doi: 10.1371/journal.pone.0192228. eCollection 2018.
Article in PlosOne: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0192228