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Dette er studietilbudet for studieår 2020-2021. Endringer kan komme.


Basic kinetics and enzyme kinetics, elementary reactions, diffusion-controlled reactions, UV-visible and fluorescence spectroscopy, fluorescence microscopy. Environmental parameters and their influence on chemical/enzymatic reactions. Single and multi-substrate enzyme kinetics, allosteric proteins, regulation of biological activity, biological rhythms. Use of spreadsheet and programming in physical and biophysical chemistry.

Learning outcome

After having completed the course the student should be able to:
  • apply the principles in kinetics/enzyme kinetics
  • calculate life time of unstable compounds on basis of first-order kinetic half-life
  • experimentally determine KM and Vmax values for enzyme catalyzed reactions
  • understand the basics of allosteric regulation of biochemical and metabolic pathways
  • estimate diffusion times
  • know the principles of an ultracentrifuge
  • determine binding constants
  • understand the principles of reversible inhibition
  • show how pH influences enzyme-catalyzed reactions
  • apply the principles of UV-VIS absorption and fluorescence spectroscopy in determining the amount of substances
  • understand the principles and applications of FRET
  • determine binding constants from isothermal calorimeter experiments
  • estimate entropy and enthalpy contributions to binding constants
  • use open access programs, such as Berkeley Madonna, for mathematical modelling of biochemical reactions and processes.

After having completed the course successfully, the student has a good competence/basis to work with a Master thesis or doing chemical, biochemical or molecular biology related research.

Contents

Basic kinetics and enzyme kinetics, elementary reactions, diffusion-controlled reactions, UV-visible and fluorescence spectroscopy, fluorescence microscopy. Environmental parameters and their influence on chemical/enzymatic reactions. Single and multi-substrate enzyme kinetics, allosteric proteins, regulation of biological activity. Use of spreadsheet and programming in physical and biophysical chemistry.

Required prerequisite knowledge

None.

Recommended prerequisites

General chemistry at university level. Knowledge within biochemistry is an advantage.

Exam

Weight Duration Mark Supporting materials
Written exam1/14 hoursA - F1)
Compilation of mathematical formulae (Rottmann).
1) Approved, basic calculator

Coursework requirements

Laboratory teaching, PC-exercises.
Compulsory course attendance that must be completed and approved before access to the laboratory: Electronic Course in Health, Safety and Environment
Mandatory lab assignments are to be completed at the times and in the groups that are assigned and published. Absence due to illness or other reasons must be communicated to the laboratory personnel as soon as possible. One cannot expect that provisions for completion of the lab assignments at other times can be made, unless prior arrangements with the laboratory personnel have been agreed.
Failure to complete the assigned labs on time or not having them approved will result in barring from taking the course exam.

Course teacher(s)

Course coordinator
Heinz Peter Ruoff
Coordinator laboratory exercises
Xiang Ming Xu
Course teacher
Heinz Peter Ruoff
Head of Department
Gro Johnsen

Method of work

Four hours lectures and six hours practical lab work each week.

Overlapping courses

Course Reduction (credits)
Physical Chemistry (MOT450_1) 10
Biophysical Chemistry (MBI110_1) 10

Open to

Biological Chemistry - Master of Science Degree Programme

Course assessment

Forms and/or discussion

Literature

Litteratur/pensum publiseres omlag 14 dager før påmelding til emnet åpner


Dette er studietilbudet for studieår 2020-2021. Endringer kan komme.

Last updated: 28.03.2020